Thatch is correct here. I submitted my TTB application 2/24/22 and this was definitely listed as a requirement, including the approved locks. I specifically stated those details in my "plant security" plan and description of doorways etc.
Since this topic has come up quite a bit, I've attached a pdf screen grab of the transcript from my TTB application. This should clear some things up and answer this question for future inquiries about it.
Tasting Room Separation.pdf